صورة الغلاف

RNA purification-free detection of SARS-CoV-2 using reverse transcription loop-mediated isothermal amplification (RT-LAMP)

Background: Current diagnosis of SARS-CoV-2 infection relies on RNA purification prior to amplification. Typical extraction methods limit the processing speed and turnaround time for SARS-CoV-2 diagnostic testing. Methods: Here, we applied reverse transcription loop-mediated isothermal amplification...

وصف كامل

محفوظ في:
التفاصيل البيبلوغرافية
المؤلفون الرئيسيون: Lai, Meng Yee, Suppiah, Jeyanthi, Thayan, Ravindran, Ismail, Ilyiana, Mustapa, Nur Izati, Soh, Tuan Suhaila Tuan, Hassan, Afifah Haji, Peariasamy, Kalaiarasu M., Lee, Yee Leng, Lau, Yee Ling
التنسيق: مقال
منشور في: BioMed Central Ltd 2022
الموضوعات:
R Medicine (General)
RV Botanic, Thomsonian, and eclectic medicine
الوصول للمادة أونلاين:http://eprints.um.edu.my/43188/
الوسوم: إضافة وسم
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الوصف
الملخص:Background: Current diagnosis of SARS-CoV-2 infection relies on RNA purification prior to amplification. Typical extraction methods limit the processing speed and turnaround time for SARS-CoV-2 diagnostic testing. Methods: Here, we applied reverse transcription loop-mediated isothermal amplification directly onto human clinical swabs samples to amplify the RNA from SARS-CoV-2 swab samples after processing with chelating resin. Results: By testing our method on 64 samples, we managed to develop an RT-LAMP assay with 95.9 sensitivity (95 CI 86 to 99.5) and 100 specificity (95 CI 78.2–100). Conclusion: The entire process including sample processing can be completed in approximately 50 min. This method has promising potential to be applied as a fast, simple and inexpensive diagnostic tool for the detection of SARS-CoV-2. © 2022, The Author(s).

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