A NADC30-like PRRSV causes serious intestinal infections and tropism in piglets
Porcine reproductive and respiratory syndrome virus (PRRSV) causes huge economic loss to China's swine industry. Currently, a novel type 2 PRRSV, called the NADC30-like strain, is epidemic in numerous provinces of China. In this study, a NADC30-Like PRRSV strain was isolated in primary alveolar...
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Main Authors: | , , , , , , , , , , |
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Format: | Article |
Language: | English English |
Published: |
Elsevier
2022
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Subjects: | |
Online Access: | https://eprints.ums.edu.my/id/eprint/34800/1/Abstract.pdf https://eprints.ums.edu.my/id/eprint/34800/2/Fulltext.pdf https://eprints.ums.edu.my/id/eprint/34800/ https://www.sciencedirect.com/science/article/pii/S0378113522000670 https://doi.org/10.1016/j.vetmic.2022.109397 |
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Summary: | Porcine reproductive and respiratory syndrome virus (PRRSV) causes huge economic loss to China's swine industry. Currently, a novel type 2 PRRSV, called the NADC30-like strain, is epidemic in numerous provinces of China. In this study, a NADC30-Like PRRSV strain was isolated in primary alveolar macrophage (PAM) cells from fecal samples collected from a local pig farm, which suffered severe diarrhea. A pathogenicity comparison study was conducted in 6–week‐old piglets by inoculating highly pathogenic HP-PRRSV and NADC30-Like PRRSV isolates. RT-qPCR revealed detection of NADC30-Like PRRSV but not the HP-PRRSV in the intestine. PRRSV infection-related lesions were observed in the intestine were further confirmed by histopathological and immunohistochemically examination (IHC). In addition, severe virus infections were also detected by RT-qPCR. Based on clinical observation and pathogenicity experiments, we confirmed that NADC30-Like PRRSV gained more tissue tropism, especially in the small intestine. This may be the one reason explaining why NADC-Like 30 PRRSV become a major epidemic strain in China since the first outbreak in 2013. |
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