Detection of Coconut cadang-cadang viroid (CCCVd) in oil palm by reverse transcription loop-mediated isothermal amplification (RT-LAMP)

• Main Authors: Thanarajoo, Sathis Sri, Kong, Lih Ling, Kadir, Jugah, Lau, Wei Hong, Vadamalai, Ganesan
• 格式: Article
• 語言: English
• 出版: Elsevier 2014
• 在線閱讀: http://psasir.upm.edu.my/id/eprint/37013/1/Detection%20of%20Coconut%20cadang-cadang%20viroid%20%28CCCVd%29%20in%20oil%20palm%20by%20reverse%20transcription%20loop-mediated%20isothermal%20amplification%20%28RT-LAMP%29.pdf; http://psasir.upm.edu.my/id/eprint/37013/

A reverse transcription loop-mediated isothermal amplification (RT-LAMP) detected Coconut cadang-cadang viroid (CCCVd) within 60. min at 60. °C in total nucleic acid extracted from oil palm leaves infected with CCCVd. Positive reactions showed colour change from orange to green in the reaction mix a...