Expression, Purification And Characterization Of Organic Solvent Tolerant Lipase From Bacillus Sphaericus 205y

• المؤلف الرئيسي: Sulong, Moohamad Ropaning
• التنسيق: أطروحة
• اللغة: English
• منشور في: 2005
• الموضوعات: Lipase; Bacillus sphaericus
• الوصول للمادة أونلاين: http://psasir.upm.edu.my/id/eprint/5905/1/FBSB_2005_6%20IR.pdf; http://psasir.upm.edu.my/id/eprint/5905/

One thousand and two hundred base pairs (bp) of open reading frame (ORF) encoding for an organic solvent tolerant lipase gene was cloned and expressed intra- and extracellularly. The intracellular expression was done using pBAD TOPO TA expression vector with 0.02% (vlv) of L-arabinose as optimum...