A novel organic solvent tolerant lipase from Bacillus sphaericus 205y: extracellular expression of a novel OST-lipase gene

• 主要な著者: Sulong, Moohamad Ropaning, Raja Abdul Rahman, Raja Noor Zaliha, Salleh, Abu Bakar, Basri, Mahiran
• フォーマット: 論文
• 言語: English
• 出版事項: Elsevier 2006
• オンライン･アクセス: http://psasir.upm.edu.my/id/eprint/7246/1/A%20novel%20organic%20solvent%20tolerant%20lipase%20from%20Bacillus%20sphaericus%20205y%20extracellular%20expression%20of%20a%20novel%20OST-lipase%20gene.pdf; http://psasir.upm.edu.my/id/eprint/7246/

An organic solvent tolerant (OST) lipase gene from Bacillus sphaericus 205y was successfully expressed extracellularly. The expressed lipase was purified using two steps purification; ultrafiltration and hydrophobic interaction chromatography (HIC) to 8-fold purity and 32% recovery. The purified 205...