Room-temperature stable loop-mediated isothermal amplification LAMP reagents to detect leptospiral DNA

• المؤلفون الرئيسيون: Lee, Pui Yuei, Wong, Yien Ping, Othman, Shuhaidah, Chee, Hui Yee
• التنسيق: مقال
• منشور في: Sciendo 2021
• الوصول للمادة أونلاين: http://psasir.upm.edu.my/id/eprint/94997/; https://sciendo.com/article/10.2478/abm-2021-0023

Loop-mediated isothermal amplification (LAMP) is one of the most promising tools for rapidly detecting Leptospira spp. However, LAMP is hampered by cold storage to maintain the enzymatic activity of Bst DNA polymerase. To overcome the drawback of cold storage requirement for LAMP reagents we modifie...