Biochemical and Microstructural Properties of Lizardfish (Saurida tumbil) Scale Collagen Extracted with Various Organic Acids

• Main Authors: Abdul Aziz Jaziri, Rossita Shapawi, Ruzaidi Azli Mohd Mokhtar, Wan Norhana Md. Noordin, Nurul Huda
• Format: Article
• Language: English; English
• Published: Multidisciplinary Digital Publishing Institute (MDPI) 2022
• Subjects: QP501-801 Animal biochemistry
• Online Access: https://eprints.ums.edu.my/id/eprint/34454/1/FULL%20TEXT.pdf; https://eprints.ums.edu.my/id/eprint/34454/2/ABSTRACT.pdf; https://eprints.ums.edu.my/id/eprint/34454/; https://www.mdpi.com/2310-2861/8/5/266/htm; https://doi.org/10.3390/gels8050266

The purpose of this research was to extract collagen from the scales of lizardfish (Sauridatumbil) using various acids. Acetic acid-extracted collagen (AScC) produced a higher yield (1.8 mg/g)than lactic acid-extracted collagen (LScC) and citric acid-extracted collagen (CScC) although not significantly different (p> 0.05). All extracted collagens were categorized as type I collagens with the presence of alpha chains (α1 and α2) based on the SDS-PAGE profiles. The triple-helical structure of the collagen was maintained in the AScC, LScC, and CScC as confirmed by the FTIR spectra. The UV-vis and X-ray diffraction spectra observed in all collagens were in agreement with previous work on fish scale and calfskin (commercial) collagens. The thermal stability of AScC (Tmax= 31.61◦C) was greater than LScC (Tmax= 30.86◦C) and CScC (Tmax= 30.88◦C). The microstructure of acid-extracted collagens was characterized as complex, fibrous, and multilayered, with irregular sheet-like structures.All samples were highly soluble in acidic pH (1.0–4.0) and in low concentrations of NaCl (0–20 g/L).In conclusion, the lizardfish scale collagen, particularly AScC, may be used as an alternative to terrestrial animal collagen.