Impact of signal peptide and transmembrane segments on expression and biochemical properties of a lipase from Bacillus sphaericus 205y

• Main Authors: Masomian, Malihe, Jasni, Azmiza Syawani, Raja Abdul Rahman, Raja Noor Zaliha, Salleh, Abu Bakar, Basri, Mahiran
• 格式: Article
• 语言: English
• 出版: Elsevier 2017
• 在线阅读: http://psasir.upm.edu.my/id/eprint/62103/1/Impact%20of%20signal%20peptide%20and%20transmembrane%20segments%20on%20expression%20and%20biochemical%20properties%20of%20a%20lipase%20from%20Bacillus%20sphaericus%20205y.pdf; http://psasir.upm.edu.my/id/eprint/62103/; https://www.sciencedirect.com/science/article/pii/S0168165617317091?via%3Dihub

A total of 97 amino acids, considered as the signal peptide and transmembrane segments were removed from 205y lipase gene using polymerase chain reaction technique that abolished the low activity of this enzyme. The mature enzyme was expressed in Escherichia coliusing pBAD expression vector, which g...