A bibliometric analysis and review of Pullulan-Degrading enzymes—Past and current trends

• 主要な著者: Kahar, Ummirul Mukminin, Ab. Latif, Nurriza, Amran, Syazwani Itri, Liew, Kok Jun, Goh, Kian Mau
• フォーマット: 論文
• 言語: English
• 出版事項: MDPI 2022
• 主題: QD Chemistry
• オンライン･アクセス: http://eprints.utm.my/id/eprint/101322/1/GohKianMau2022_ABibliometricAnalysisandReviewofPullulanDegrading.pdf; http://eprints.utm.my/id/eprint/101322/; http://dx.doi.org/10.3390/catal12020143

Starch and pullulan degrading enzymes are essential industrial biocatalysts. Pullulan-degrading enzymes are grouped into pullulanases (types I and type II) and pullulan hydrolase (types I, II and III). Generally, these enzymes hydrolyse the a-1,6 glucosidic bonds (and a-1,4 for certain enzyme groups) of substrates and form reducing sugars such as glucose, maltose, maltotriose, panose or isopanose. This review covers two main aspects: (i) bibliometric analysis of publications and patents related to pullulan-degrading enzymes and (ii) biological aspects of free and immobilised pullulan-degrading enzymes and protein engineering. The collective data suggest that most publications involved researchers within the same institution or country in the past and current practice. Multi-national interaction shall be improved, especially in tapping the enzymes from unculturable prokaryotes. While the understanding of pullulanases may reach a certain extend of saturation, the discovery of pullulan hydrolases is still limited. In this report, we suggest readers consider using the next-generation sequencing technique to fill the gaps of finding more new sequences encoding pullulan-degrading enzymes to expand the knowledge body of this topic.